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Cell Biology

Categories
+ Cell engineering
+ Cell imaging
+ Cell isolation and culture
+ Cell metabolism
+ Cell movement
+ Cell signaling
+ Cell staining
+ Cell structure
+ Cell Transplantation
+ Cell viability
+ Cell-based analysis
- Model organism culture
Embryo selection
Embryoid body
Maintenance
+ Organelle isolation
+ Single cell analysis
+ Tissue analysis
Protocols in Past Issues

Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells Using Hanging Droplets and Retinoic Acid
JW Jeroen Witteveldt
SM Sara Macias
Q&A 0 Q&A
7169 Views
Nov 5, 2019
Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.